FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005326324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005326324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11631540
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG120040.10320215551852978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG37250.027.047022
TACTCGG42250.023.3323233
AAAGTGC198150.022.3512734
CGAGTAG33350.021.7345163
GCTACTC97150.021.3435251
CCAAAGT232000.020.838612
AGTAGCT130450.020.5043875
CCGAGTA36500.020.4538572
AAGTGCT223900.019.9430275
CCCAAAG275350.019.6951561
TAGCTGG151250.019.5067947
GCTGGGA383600.019.5040749
CAAAGTG244650.018.4877973
GAGTAGC90350.017.4095174
GTAGCTG156250.016.6550676
AGTGCTG274600.016.4459696
GTCTCGC47300.015.020665145
ACTCGGG69100.014.47625354
GGAGGCT318750.014.4199359
TCGGGAG73950.014.11556
Produced by FastQC (version 0.11.5)