FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005326326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005326326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11977432
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG39900.025.6240022
CGAGTAG37750.022.8576093
TACTCGG46350.022.2146453
AAAGTGC197300.021.9405774
CCGAGTA42250.021.2811812
CCAAAGT228150.020.7853852
AGTAGCT130600.020.0986185
TAGCTGG152000.019.937227
GCTACTC96900.019.8302571
AAGTGCT226100.019.2420565
CAAAGTG237650.019.1611943
CCCAAAG291050.018.8846041
GCTGGGA415550.018.5457219
GAGTAGC92100.017.8717484
GTAGCTG161650.016.8658226
GTCGAGA27600.016.0260681
GCTACTT85250.015.6505521
AGTGCTG291250.015.1118666
CTACTTG79200.014.831692
ACTCGGG77100.014.2011444
Produced by FastQC (version 0.11.5)