FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005326328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005326328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13554074
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG38700.021.9214953
AAAGTGC220300.021.130954
CTACTCG43900.020.9767252
CCAAAGT247450.020.0725062
GTCGAGA31650.019.2478681
GCTACTC109100.019.0115831
TACTCGG51550.018.707613
AAGTGCT252950.018.6328285
CCGAGTA48650.018.4815042
CAAAGTG264900.018.4216423
AGTAGCT137650.017.8049435
CCCAAAG326700.017.4703921
GCTGGGA474600.016.9105429
TAGCTGG166300.016.392237
GAGTAGC98550.015.8190474
AGTGCTG332550.014.4124626
CTACTTG86150.014.3084352
AGGATCG68100.014.1602145
GTAGCTG179950.013.9820656
TCTCGTT49900.013.803458145
Produced by FastQC (version 0.11.5)