FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005326329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005326329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13554074
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG153430.11319843760628724No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG42550.023.5117932
AAAGTGC227750.023.1713854
CGAGTAG43000.021.7470463
CCAAAGT267300.021.6155172
AGTAGCT137300.021.2766845
AAGTGCT256050.020.6948385
CAAAGTG274100.020.3638883
GCTACTC104650.020.2395841
CCCAAAG337750.019.4791451
TAGCTGG170100.019.4761077
CCGAGTA50350.019.1495442
GCTGGGA474500.018.9751519
GAGTAGC101050.018.579864
TACTCGG51050.018.4597843
CGCGATA8600.016.86029145
GTAGCTG183750.016.4116176
AGTGCTG342900.016.2148636
CTACTTG90450.015.629042
GCTACTT98100.015.2319741
TACTTGG110600.014.0916663
Produced by FastQC (version 0.11.5)