FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005326333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005326333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11842238
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA9450.024.54932145
AAAGTGC195450.023.2462674
CCAAAGT221650.021.9399092
CTACTCG36750.021.6927872
CGAGTAG38450.021.1064663
AGTAGCT120650.020.9633965
AAGTGCT216750.020.8301535
CAAAGTG233250.020.6582643
CCGAGTA43950.019.6231232
TACTCGG42800.019.2998943
GCTACTC94350.019.2974131
CCCAAAG286400.019.1223491
TAGCTGG147800.019.0785397
GCTGGGA409250.019.0258589
GAGTAGC89250.018.6741564
GTAGCTG153550.016.6620646
AGTGCTG287800.016.2432466
GTCTCGC61200.014.925897145
CGACGTA11700.014.25159145
GCTACTT85050.014.2432611
Produced by FastQC (version 0.11.5)