FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005326336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005326336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12449461
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG39000.022.682472
CGAGTAG38650.021.1993373
AGTAGCT133550.021.1203355
AAAGTGC209550.020.8652234
GCTACTC100050.020.367861
TACTCGG43800.020.3621853
TAGCTGG151500.019.7160727
CCGAGTA40450.019.3597772
CCAAAGT234700.019.3399962
AAGTGCT236350.018.59145
CTACTTG84400.018.2992082
CAAAGTG249800.018.0257173
GCTGGGA427500.017.925649
GCTACTT90050.017.8782671
CCCAAAG294100.017.7292371
GAGTAGC94250.017.4637644
GTAGCTG164800.016.6752996
TTGGGAG232300.014.6703436
TACTTGG105850.014.6594033
AGTGCTG308400.014.3419136
Produced by FastQC (version 0.11.5)