FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14118
Sequences flagged as poor quality0
Sequence length49-150
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACGGTGATCTCGTAT1641.1616376257260235TruSeq Adapter, Index 3 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGG100.009310952131.911351
TACTATG100.009310952131.911359
AAGGGGG400.0088891917.73407770-74
AAAAAAA3951.4273293E-45.656137565-69
Produced by FastQC (version 0.11.5)