FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435314
Sequences flagged as poor quality0
Sequence length49-150
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC2155.031968E-513.264801135-139
CGGGGGG3353.3526576E-612.4706140-144
GGTCGCC3101.4202487E-712.362335130-134
TGGTCGC3101.4202487E-712.362335130-134
GCCGTAT2451.705592E-411.64054135-139
GGGGGCG3102.8202336E-411.026058140-144
CGTATCA2550.00924407810.42551140-144
GTCGCCG2951.6530407E-410.299293140-144
TCGCCGT2500.002629834210.140204135-139
CGCCGTA2500.002629834210.140204135-139
GTGGTCG3203.359891E-510.133549130-134
CGGTGGT3554.7508518E-59.818859135-139
TCGGTGG3554.7508518E-59.818859135-139
CTCGGTG3106.129893E-49.8009405140-144
GATCTCG3551.04718245E-49.134467130-134
AGGGGGG4050.00189455818.439699140-144
AAGGGGG4600.00111400078.2562275140-144
CACAAAT6050.0061813547.8599941
GGGGGGG112250.07.3758082140-144
GGTGGTC4500.00134208297.20608130-134
Produced by FastQC (version 0.11.5)