FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2683
Sequences flagged as poor quality0
Sequence length49-150
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACGGTGATCTCGTAT42915.989563920983974TruSeq Adapter, Index 3 (97% over 38bp)
GATCGGAAAGCACACGTCTGAACTCCAGTCACTTACGGTGATCTCGTATG140.5218039508013418TruSeq Adapter, Index 3 (96% over 29bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACGGTGATGTCGTAT110.40998881848676855TruSeq Adapter, Index 3 (97% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTTACGGTGATCTCGTATGCC90.3354453969437197TruSeq Adapter, Index 3 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACGGTGAGCTCGTAT60.22363026462914648TruSeq Adapter, Index 3 (97% over 38bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN40.14908684308609765No Hit
ATCACTAATACCTACCTTAATACTACACAAGAAGTATATTAGGATTAGGA30.11181513231457324No Hit
AGAAAAATGAATTCTTGATGCAGTGCTCAGCTCTAGAGTTGATCCTGAAT30.11181513231457324No Hit
CAAGAGGACCCTTTCAAGGTGTCATAGCCAAGGAGAGGAGTGTGTTGTTT30.11181513231457324No Hit
CCAAGGTTAAGCACTTGCTCAAGGTCTCCTAGCTGGTGAGCAACAGAGCT30.11181513231457324No Hit
GTACCTTTATACTTGGGTTCAAACCTTTCTGAAAACCAGAGTTTTCAAGG30.11181513231457324No Hit
GGCGAAATTATTAGCCATATTCCCAAGCCAGGCACTATGAGCTGAGTTGT30.11181513231457324No Hit
CTCCTATGTGAAATGGGTAGAAAACCTGTCTTCTCAGGGCTGTCGTAAGG30.11181513231457324No Hit
TTTCTCTGCAACCTCTCCAACATTTGTTATTTTTTGACTTTTTAATAATA30.11181513231457324No Hit
CCAGGTTCTATAAGAAACATGACTGATTCTTTATAATAACCACTCTGAAT30.11181513231457324No Hit
CAGTTCCTTCATGTCCACCAAAGTACCCGTCTCACCATTTACACCCCAGG30.11181513231457324No Hit
ATCTGTTGCAATTTTTATTTGTTTTATTTTTGAAGTAATTTTGGGTTTAC30.11181513231457324No Hit
CATCCCTGGGATACCAATCTCCTTTTTCAAAGGGCTTTTCACAGGGACCC30.11181513231457324No Hit
GACCTGTCTCTAAGAAGAAAACAAACCAAAACAAACAAATAACAAAAAAA30.11181513231457324No Hit
TGAGAAGTACAGTTTAAATATAAATTACCAAGAATATTCTTTTCTACAAT30.11181513231457324No Hit
CACAGCGCCTGCCTTGTGCGCAGTCAGCACGTGTGGGGCAGGGCCGTTGC30.11181513231457324No Hit
AACAGTATGAAAAGTTCTTTGTCAGTTGTGATTCACTCTGGGTAGGACAT30.11181513231457324No Hit
CTATGTCCAAATATAGGAGGATGTATTAGTTTGCTAGGGCTACCATAAGAAATACCACA30.11181513231457324No Hit
GGGCAGGGTGCTCAGGTCACCGCAGGGGGACGACAAACCGAGTTGGCTCC30.11181513231457324No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTAAT100.007850816137.509431
GATCGGA500.0137.509431
TCGGAAG500.0137.509433
GAGCACA500.0137.509439
CGGAAGA500.0137.509434
ATCGGAA500.0137.509432
AAGAGCA550.0125.008587
GAAGAGC550.0125.008586
AGAGCAC550.0125.008588
GGAAGAG550.0125.008585
AAAAGGG355.167216E-627.50188870-74
ACACGTC503.5506673E-927.50188610-14
GGTGATC503.5506673E-927.50188635-39
GTATGCC503.5506673E-927.50188645-49
ATCTCGT503.5506673E-927.50188640-44
CAGTCAC503.5506673E-927.50188625-29
ACGTCTG503.5506673E-927.50188615-19
TGCCGTC503.5506673E-927.50188650-54
CCAGTCA503.5506673E-927.50188625-29
TACGGTG503.5506673E-927.50188635-39
Produced by FastQC (version 0.11.5)