FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences923976
Sequences flagged as poor quality0
Sequence length49-150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGC4303.6379788E-1213.00025140-144
GGTCGCC4002.3610482E-911.768647140-144
GTCGCCG3558.456045E-710.774114140-144
GCCGTAT3406.690441E-59.518758140-144
GTGGTCG4804.254598E-79.194256140-144
CGCCGTA3200.00348732278.27483140-144
TCGCCGT3300.0047078.024077140-144
CGGTGGT5101.2355249E-67.843207135-139
CAATTAG6650.0063956897.83204461
GATCTCG5301.5857227E-57.7717476140-144
CGTATCA3450.0076729997.675205140-144
GGGGGGG134350.07.2705455140-144
TCGGTGG4551.5119024E-47.1429205135-139
CTCGGTG4702.2523067E-46.914955135-139
CCCAGCT15351.2165656E-66.7860721
ATCTCGG5955.4840074E-46.4282527140-144
CCCCAGC10000.00494624076.36571171
CCAGAAT12209.050218E-46.16648671
GGTGGTC7552.495447E-45.8453546140-144
GCTGAGG12300.0066808415.64403157
Produced by FastQC (version 0.11.5)