FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20228172
Sequences flagged as poor quality0
Sequence length49-150
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTCG7301.0786607E-97.332116140-144
CGCCGTA44850.06.9010243140-144
GTCGCCG48250.06.6558905140-144
AAGCGCG15050.06.3397436140-144
GCGTGAG93100.06.29997252
CGTGAGC92750.06.1216973
GCGCGTC8203.4486493E-65.6759753140-144
GCCGTAT52650.05.569248140-144
TCGCCGT45450.05.525291135-139
GGCGTGA111000.05.4531691
CCGTATC53600.05.4077916140-144
TGGTCGC55700.05.264289140-144
ACCGGTG91300.05.2507434140-144
GTGGTCG61950.05.1839657140-144
GGTCGCC56600.05.1112256135-139
TACTCGG32151.7916318E-75.0452047
Produced by FastQC (version 0.11.5)