FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences518963
Sequences flagged as poor quality0
Sequence length49-150
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGC250.007124948352.17179140-144
CGGCGGG650.00899877526.754763140-144
GGCTGCG900.001168907725.204674135-139
GGGGGGG81350.015.124581140-144
ACACCTA2200.006673077613.7525082
GGCGGGG2804.8161714E-412.421855140-144
AGGGGGG4552.2433289E-510.510799140-144
TATGCCG4304.42112E-58.6511135125-129
ATGCCGT4002.2171294E-48.524951125-129
TGCCGTC4059.966756E-48.350165130-134
TCGGAAG7004.230904E-67.831754135-139
TCTCGTA3457.028325E-47.6581736110-114
CTCGTAT4004.2638544E-47.608386140-144
CGTATGC4600.0061084987.5611286140-144
CCGTCTT4050.0092505177.515149130-134
AAGGGGG5800.00288368627.4959464140-144
CGTCTTC4250.00106912337.3623824120-124
GTATGCC5150.00130850417.223298130-134
AAAGGGG7901.4763103E-47.1543593140-144
CGGAAGA7255.6636156E-57.0575805135-139
Produced by FastQC (version 0.11.5)