FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329674

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329674
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1636673
Sequences flagged as poor quality0
Sequence length49-150
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCGTG1550.00983534317.4554444
GGGGGGG275000.015.684985140-144
TCTCGTG2100.003076396215.4558137
CATATCG2406.0347338E-513.012986140-144
AGGGGGG16650.012.088139140-144
CACACGA3650.00954396910.382381
ATCGTAT2700.00234724410.281866140-144
AAGGGGG20700.09.555429140-144
TATGCCG31250.09.438752140-144
GCCGTCT29400.09.088434140-144
ATGCCGT30400.08.789473140-144
TGCCGTC29900.08.472222140-144
CCGTCTT28850.08.299442140-144
GGGGGCG4301.0316287E-48.148234135-139
CGTCTTC27500.08.075938140-144
AAAGGGG26650.08.073097140-144
CGTATGC32750.07.8409033140-144
TCGTATG32900.07.8051543140-144
CCCATAT8351.7631067E-47.78012371
GTATGCC35850.07.7436647140-144
Produced by FastQC (version 0.11.5)