FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329675

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329675
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1636673
Sequences flagged as poor quality0
Sequence length49-150
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGA1050.003963085414.879398125-129
AAGCGTC1850.00125069113.624466140-144
CGCCGTA8250.010.592472135-139
TCGCCGT8300.010.165606135-139
CGTATCA9600.010.127113140-144
GCCGTAT8850.09.7647505140-144
CCGTATC7950.09.476024135-139
GTCGCCG8600.09.460597135-139
GGGGGGG258700.09.381162140-144
CGGGGGG9101.0913936E-119.100801140-144
AGCGGGG3600.00182077159.001879140-144
TGGTCGC9900.08.82706135-139
GTGGTCG10250.08.525648135-139
GGGCGGG8802.9236617E-88.479138140-144
AGGGGGG13700.08.410515140-144
GGTCGCC9350.08.378321130-134
GGGGGGC9904.8567017E-97.6379576140-144
TGGGGGG18050.07.580529140-144
CTCCAGC19951.8189894E-127.53118941
GGGGGCG7451.2663406E-57.249835140-144
Produced by FastQC (version 0.11.5)