FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences783515
Sequences flagged as poor quality0
Sequence length49-150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAAG800.003178218133.10278135-139
CGCCCAC1200.004344337620.108253140-144
GGGGGGG138050.011.081752140-144
AGGGGGG6151.4362195E-710.985973140-144
ATGCCGT6002.9594958E-910.55314130-134
CTCGTAT6001.0422809E-910.294101125-129
TGGTTCC4050.004664429.4616114
TCGTATG5857.98791E-89.384935125-129
CGTATGC6352.265578E-59.119964140-144
AAGGGGG7602.5987338E-68.889964140-144
AAAGGGG10353.6834535E-98.859288140-144
TGCCGTC5501.0819165E-58.803689130-134
CCGTCTT6001.1542179E-68.578418125-129
TCTCGTA6151.6448466E-68.369188125-129
CTCACAT6302.7201622E-48.364991
GCCGTCT5402.476721E-58.276973130-134
TATGCCG5908.49832E-68.206829130-134
CCCAGAC5400.00537603677.98476271
TGCTTGA10001.4863654E-67.8269835140-144
CGTCTTC6201.657613E-47.820722135-139
Produced by FastQC (version 0.11.5)