FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1115568
Sequences flagged as poor quality0
Sequence length49-150
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTTCATAGATCTCGTAT12020.10774780201655121TruSeq Adapter, Index 27 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT350.00862851226.98973140-144
GGCACGA1500.003363628421.017311
CGGAAGA9550.020.4616134
CACCGTT1600.00488769919.7037261
TCGGAAG9950.019.6390343
AGAGCAC15600.013.7371598
GAAGAGC15350.013.5514926
GAGCACA15200.013.2693299
AAGAGCA22950.09.8869527
GGAAGAG23050.09.02452855
GGGGGGG241550.07.13711140-144
TCTCGTA21200.07.017966140-144
CTCGTAT21000.06.9723463140-144
CGTATGC20600.06.8784504140-144
CAAAGGG11158.4637577E-46.78585771
ATCTCGT21450.06.743918140-144
TATGCCG19200.06.5190034140-144
TCGTATG21100.06.379681140-144
GATCTCG22250.06.368363140-144
TGCCGTC18450.06.2720037140-144
Produced by FastQC (version 0.11.5)