FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1115568
Sequences flagged as poor quality0
Sequence length49-150
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA2552.2375469E-510.51669140-144
GTCGCCG3007.5187945E-69.081443135-139
GGTCGCC3556.9727103E-68.264801135-139
TCGCCGT2950.00110644388.264271140-144
CTAAGAT9251.1019987E-48.1009631
GGGGGGA7300.00747359127.69730762
CCGTATC3306.2831055E-47.3877573140-144
GTGGTCG3751.2271965E-47.265154135-139
TCGGTGG3759.6944335E-46.7062955135-139
GCCGTAT3300.0047928666.6489816140-144
CGTATCA4059.3782315E-46.6216197140-144
CCCAGCT14204.7310918E-56.5962941
CTTGAAC10700.0045322156.4195711
AACAAGG11350.00839470056.04976036
CAATGAA17802.6703005E-55.96384051
CGGTGGT4100.00437634765.8647294130-134
TCTCGGT4500.00193637975.7887053130-134
CTGAAAG16253.0136894E-45.76414631
CCCAGCC15208.043242E-45.7515061
CAAAAGG18452.8437126E-45.41527751
Produced by FastQC (version 0.11.5)