FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences898323
Sequences flagged as poor quality0
Sequence length49-150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATCC350.00908856349.057837
CCCGTAT850.00870374726.9397095
GGGGGGG105300.010.256761140-144
CGTATGC6951.1150405E-98.804587140-144
GCCGTCT5902.960769E-87.8855367130-134
TCGTATG7001.0367512E-77.8675284140-144
CCGTCTT6401.535227E-87.652083130-134
AGGGGGG6107.501789E-67.523593140-144
CTCGTAT7251.5102269E-67.1742215140-144
TATGCCG6057.838058E-67.0800533140-144
TGCCGTC6051.0770964E-67.0800533140-144
CGTCTTC6602.1270534E-77.049191130-134
CGGGGGG4900.00223257536.8684773140-144
ATGCCGT6402.716246E-66.692863140-144
TATCTCG7101.9498711E-66.5958505135-139
TCTCGTA7202.9228942E-45.949211140-144
GTATGCC8501.1789514E-45.759237140-144
CTCAAAT12000.0056746515.7278771
CACACGT11655.0786184E-95.675021130-134
ACACGTC9704.737176E-65.3012366130-134
Produced by FastQC (version 0.11.5)