FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences898323
Sequences flagged as poor quality0
Sequence length49-150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA3407.255512E-712.362382140-144
GATCTCG5501.4551915E-1111.019992140-144
GCCGTAT4356.589471E-810.449992140-144
TCGCCGT3401.21696256E-410.301985140-144
CCCAAGC6052.7805628E-49.49021151
TGGTCGC3801.1111752E-69.466304135-139
TCTCGGT5703.7425707E-89.038327140-144
CGTATCA4055.822183E-68.979253140-144
TCGGTGG5401.5778096E-78.979253140-144
GAGCCCC5300.0096100038.6646044
GGTCGCC3901.5748292E-58.564752135-139
GTGGTCG4204.3131768E-68.564752135-139
CCGTATC3701.6986027E-58.513502130-134
GTCGCCG3653.1260075E-58.447426135-139
CTCGGTG5401.5155492E-68.41805140-144
CGGTGGT5804.697231E-78.359994140-144
ATCTCGG6301.88189E-78.177533140-144
GTAGATC8302.5465852E-107.4296637135-139
CACCAAG8150.0052877817.0448821
GGTGGTC7452.6930775E-66.91523140-144
Produced by FastQC (version 0.11.5)