FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44769
Sequences flagged as poor quality0
Sequence length49-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCTTTATATCTCGTAT22374.996761151689785TruSeq Adapter, Index 11 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2150.0133.09421
ATCGGAA2150.0133.09422
CGGAAGA2200.0126.89694
TCGGAAG2250.0124.0769653
AGAGCAC2400.0116.322178
GAGCACA2550.0109.4796759
GAAGAGC2600.0107.3743066
AAGAGCA2750.0101.5175257
GGAAGAG2900.096.266615
TATGCCG2050.027.3096445-49
ATGCCGT2100.026.6594145-49
ACACGTC2200.026.6483510-14
TCTCGTA2100.026.64746940-44
TATCTCG2050.026.61497340-44
CCGTCTT2150.026.13311250-54
CACGTCT2300.026.09662210-14
TGCCGTC2100.026.08644750-54
CTCGTAT2150.026.02776140-44
CGTCTGA2200.026.01386515-19
ATCTCGT2100.025.98128340-44
Produced by FastQC (version 0.11.5)