FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44769
Sequences flagged as poor quality0
Sequence length49-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG760.1697603252250441No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGCG100.007886106139.786353
TCCGGAA100.007912534139.630179
GAGTCCG100.007912534139.630176
TGACGGT100.007912534139.630179
GTCCGGA100.007912534139.630178
CAGTGTA300.00223423569.815099
GGGGAGT551.5601696E-417.90313560-64
AAGGGGG1302.1960659E-814.24310580-84
AAAAGGG1201.3149838E-714.21071275-79
AATGGGG800.00243363312.46271680-84
AAAAAAG3800.011.96691475-79
AAAGGGG1357.4189593E-611.60549380-84
ATGGGGG1009.900753E-411.39448480-84
AAAAAGG2003.972673E-911.36856975-79
GGGGGAG1903.6004424E-411.0357643
GGGGGGA2602.7512215E-810.7527962
AGGGGGG2807.471135E-88.64759980-84
TAAAAAA4707.2759576E-127.827421770-74
AAAATGG1650.00697543477.75129775-79
AAAAAAA27650.07.36903570-74
Produced by FastQC (version 0.11.5)