FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences737982
Sequences flagged as poor quality0
Sequence length49-150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCGG200.003943555560.585583140-144
AACGGGA950.00491250330.284603125-129
GTCGCCG3752.7866918E-814.002001140-144
GGTCGCC4108.967618E-812.806708140-144
TCGCCGT3451.1026051E-412.198754140-144
TCGCTGT7151.1277734E-1010.73311140-144
CGTATCA3951.3214722E-610.405577130-134
TGGTCGC3900.00416574079.592182140-144
CGCCGTA3406.894414E-59.503621140-144
CAAGCCT4350.00590746669.1938881
GTGGTCG4454.3462816E-49.076491140-144
CGCTGTG7151.4227408E-79.038408140-144
CCGTATC3555.2543514E-48.906182130-134
GATCTCG6453.140356E-68.766907140-144
ATTCGCT7102.1020242E-88.643633135-139
ATCTCGG6705.2442847E-68.439783140-144
TCATTCG7001.53761E-78.28005135-139
GCGGGGG4900.00113328128.242937140-144
CATTCGC7152.1607775E-78.106344135-139
AGGGGGG10005.5806595E-98.078078140-144
Produced by FastQC (version 0.11.5)