FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1886639
Sequences flagged as poor quality0
Sequence length49-150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTCCG2950.006026291313.96853351
CTACTCG3950.002927946412.1670236
CTCGGGA5853.235247E-511.7355799
CTCCAGC39650.07.27491141
CTCAAAA58300.07.1859281
GCTACTC14551.7719402E-57.07820565
CTCACAC20853.2699973E-86.91727071
CTCTAGT14209.7864766E-56.77113151
CTCCCAA43800.06.5856211
CCCTTGA13654.4416956E-46.5408211
CTTTAAT33651.8189894E-126.5311021
ACAGGCG14801.6868305E-46.49421559
CTCAAGT22351.2252167E-76.4530251
CCCCCTT14207.171055E-46.28747941
TCCAGCC39400.06.27420572
CTCACAT21102.0408115E-66.1843311
CTCAAAT29904.0326995E-95.9720541
CTCCCAG28851.1512384E-85.95135351
CTTCAAG22305.230908E-65.85154151
CTCAGGT22405.6424633E-65.8254191
Produced by FastQC (version 0.11.5)