FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23857
Sequences flagged as poor quality0
Sequence length49-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACGGTGATCTCGTAT13375.604225175001048TruSeq Adapter, Index 3 (97% over 38bp)
GATCGGAAAGCACACGTCTGAACTCCAGTCACTTACGGTGATCTCGTATG480.20119880957371003TruSeq Adapter, Index 3 (96% over 29bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACGGTGATGTCGTAT420.17604895837699627TruSeq Adapter, Index 3 (97% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTTACGGTGATCTCGTATGCC290.1215576141174498TruSeq Adapter, Index 3 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCTCC100.00786428139.779888
GCCTCGG100.00786428139.779884
AGAGCAC1750.0131.792478
TCGGAAG1700.0131.557533
GATCGGA1850.0128.446371
CGGAAGA1750.0127.798754
ATCGGAA1950.0125.443482
GAGCACA1900.0121.387789
GAAGAGC1850.0120.890696
AAGAGCA1950.0118.275287
GGAAGAG2200.0101.658095
CGGAAAG300.002211975969.889944
TCGGAAA300.002211975969.889943
GAAGGCA300.002211975969.889949
ACCAGAA350.004074800359.9056635
CGTCTTC1900.027.25457250-54
TATGCCG1900.027.22029145-49
GGTGATC1950.026.52233735-39
TTACGGT1950.026.52233730-34
ATGCCGT1950.026.52233745-49
Produced by FastQC (version 0.11.5)