FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23857
Sequences flagged as poor quality0
Sequence length49-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG730.305989856226684No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGGGG658.658527E-442.9666188
GAGGGGG700.001246833239.897579
GGGAGGG850.003233984832.8568237
GGGGGAG1102.9511406E-431.7367065
GGGGAGG900.00427856731.0314466
GGGGGGA1654.4983608E-816.9262434
AAGGGGG602.8138107E-416.5623480-84
AAAGGGG600.005373649314.19629180-84
GGTTTTT700.001028318113.9641530-34
AAAAAAG1851.1878001E-912.25697575-79
AGGGGGG1202.9246976E-511.83024380-84
AAAAAGG900.00606338811.02276575-79
AAAAAAA16150.010.05310670-74
TAAAAAA2653.3483593E-78.52411170-74
AAAAAAT2303.543769E-58.01033475-79
TTTTTTT5801.0537615E-55.055985540-44
Produced by FastQC (version 0.11.5)