FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329703

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329703
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15687
Sequences flagged as poor quality0
Sequence length49-150
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG400.2549882067954357No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGGGT100.00843845136.37383
ACGAAGA100.00843845136.37383
GTAATTT300.002423555668.18691
AATTGAT350.00446404658.4459155
AAGGGGG403.579686E-421.25747180-84
GGGGGGA750.00186669518.1831744
AAAAAGG655.537344E-415.14112875-79
AGGGGGG904.203331E-412.59701980-84
AAAAAAG1502.1762517E-611.24769475-79
AAAAAAA10800.07.381452670-74
TTTTTTT3450.0069746065.6026254135-139
Produced by FastQC (version 0.11.5)