FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences270669
Sequences flagged as poor quality0
Sequence length49-150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATATATATATATATATATATATATATATATATAT8820.3258592598339669No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGC300.00826847427.223953115-119
CCCATGC1500.006714496318.6438051
GGGGGGG79350.014.049869140-144
GCGGGGG1400.002198786512.63819135-139
CTCCAGG3150.00248589712.4292021
CTCAAAA6809.21209E-710.692771
CTTTCAG3750.00912408810.440531
GATTACA5150.0093903458.6851635
TGGGGGG3350.00617642477.8612556140-144
AGGGGGG4002.3648824E-47.582914135-139
CTAAAAA6700.0092334647.51317451
TCTCGTA5908.7419085E-67.4393234140-144
TCAAAAA8100.00639941786.90383632
TCGTATG6008.729717E-56.8276463140-144
CGTATGC6059.73132E-56.7712193140-144
AAGGGGG4200.00327971116.6200047135-139
GCTTGAA6352.7901988E-66.4508343125-129
AAAGGGG5450.00151888536.442864140-144
ATCTCGT5700.00251578426.160282140-144
AAAAGGG5854.2055847E-46.0490484135-139
Produced by FastQC (version 0.11.5)