FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1101904
Sequences flagged as poor quality0
Sequence length49-150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG4500.024.5068863
CGGAAGA5200.021.207884
TTGCGCA750.001544956613.2484280-84
AAAACGA4150.004112770311.6260064
AGAGCAC11554.1836756E-1111.3368698
CTCAGAC7603.9775852E-59.9801521
GAAGAGC13608.203642E-109.6293136
GATCGGA11556.085611E-89.5520381
GAGCACA13054.127287E-99.5056879
CCCAACT10251.1891407E-69.4180771
ATCGGAA12351.7146522E-78.9296342
TAATGGG8751.8600131E-48.6649344
CTCAAAT18553.6743586E-108.1778071
GTTAAAC9404.013777E-48.0690591
CCCAGAG13809.234009E-77.99464131
CTGCAAT11505.11734E-57.79477451
TAAATCC9755.967658E-47.77622273
CTAAAGG9500.0039567377.2582921
GGAAGAG23005.16593E-107.19223745
CCCAGAT10650.00152127337.1219871
Produced by FastQC (version 0.11.5)