FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1101904
Sequences flagged as poor quality0
Sequence length49-150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGTT2750.003722277515.0190211
CGAAAAG3100.00823785113.3233241
GGTCTCG4850.00102645611.3530393
ATATTCC13154.5704655E-78.3741056
CTGAGAT17651.2405508E-98.1902591
CTCAGGG13405.3394742E-67.70565371
AATCTGC9907.1371545E-47.64718685
CTGAAAT25251.8189894E-127.63342951
CTTTTGC16351.940698E-77.5784051
CCCATGC8300.0098348597.46427251
CTGCACT17658.84138E-87.4102351
ATCTGCC11655.1385845E-47.0892376
CACCAAT9800.00544323737.0242021
AATGGGG11350.00303541226.67023326
CTTGAAT14501.2459407E-46.64634851
CTTGAAC15905.874427E-56.4940731
TTGAACC11850.00475836646.3896592
CCCAAAT18409.913387E-66.35995671
AATCCAA15502.967477E-46.2164235
CTGCTGT14650.00101483566.1084181
Produced by FastQC (version 0.11.5)