FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1636851
Sequences flagged as poor quality0
Sequence length49-150
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTGA905.403799E-737.1969539
ACGGCGG804.955566E-429.196442140-144
GGGGGGG439650.024.119522140-144
CAGCGCG1150.004101668520.31057140-144
ATCAGCG1800.005062435819.584396140-144
TGTCGTA1701.632287E-518.48065135-139
AGGGGGG27500.018.006243140-144
TCAGCGC1850.001306300317.569164140-144
CAGCTCG3401.3739227E-615.932821140-144
ATGCCGG2058.125855E-515.325418135-139
GCTCGTA2501.00461126E-414.948579140-144
GCGTATG2501.00461126E-414.948579140-144
CATGTCG1856.486719E-414.859441135-139
AAGGGGG31500.014.82994140-144
ATGTCGT1800.0092588213.09046135-139
CGTATGA2500.001700351213.080007140-144
AAAGGGG38200.011.372855140-144
AGCTCGT3050.007521237210.721317140-144
CGTCTTC40950.09.582422140-144
AAAAGGG44550.09.437234140-144
Produced by FastQC (version 0.11.5)