FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences620908
Sequences flagged as poor quality0
Sequence length49-150
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG250.0061773243151.7964140-144
TCGCGGG250.001047439484.62031135-139
CGCGTCG350.001837385473.44632140-144
TCGCGGT400.003119931664.265526140-144
ATGTCGC300.00329974663.367256130-134
CGGGTGG800.004869099257.42298130-134
CGGGGGT1158.623989E-636.791443135-139
GGGGGGG119050.030.517805140-144
AGGGGGG6150.026.47252140-144
GCGGGGG2702.147317E-723.50564135-139
TGGTCGC5407.2759576E-1222.215244140-144
GGGGGCG2403.430867E-421.421843140-144
GGTCGCC5251.09139364E-1021.217827140-144
TGGGGGG7100.020.516695140-144
GTGGTCG5552.3101165E-1020.070917140-144
CGTATCA5653.6379788E-1219.71568140-144
AAGGGGG5804.1836756E-1019.205791140-144
CCGTATC5351.8189894E-1218.950954130-134
GGGGGGC2350.00764106818.231356140-144
TCGCCGT5000.018.159077125-129
Produced by FastQC (version 0.11.5)