FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382113
Sequences flagged as poor quality0
Sequence length49-150
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATATATATATATATATATATATATATATATATAT37890.9915914925689521No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATA4700.12300026431971693No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGCG1750.001498778217.207308140-144
GCGGGGG1750.003856242414.93625140-144
CGGGGGG1809.212928E-414.18329135-139
GTCGCCG3155.9509093E-613.82986140-144
CCGTATC2952.2214928E-413.610298140-144
GGGGGGG106900.013.285205140-144
CGTATCA2651.8612684E-413.151415140-144
GCCGTAT3056.740207E-512.854969140-144
TGGTCGC3455.2831274E-712.685676135-139
AGGGGGG3256.104983E-712.535743130-134
CGCCGTA2952.590377E-411.813983140-144
GGTCGCC3406.211838E-611.799543135-139
CGGTGGT3902.3207976E-611.221943135-139
GGGGGGA2300.00580096711.099966135-139
GTGGTCG3601.4370221E-410.891015140-144
TCGCCGT2856.9704105E-410.6999445140-144
CTTCTAA3550.001742408110.6525991
TGGGGGG7253.274181E-1110.215021140-144
CACCGGT3950.00284536429.918808140-144
GGGGGGT4854.422968E-59.880509140-144
Produced by FastQC (version 0.11.5)