FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2895
Sequences flagged as poor quality0
Sequence length49-150
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATATATATATATATATATATATATATATATATAT140.48359240069084625No Hit
TCCTGTCTTTGTCTTTTAAATAAGTCAAAGGCCCACAGAAACAAGAAACC40.1381692573402418No Hit
TGTTGGGAAATGAAAGGATATTATGTTGGAAGGGTAGAGTGAAGTTATAT30.10362694300518134No Hit
GCTTTAATCCAGAAAGCAGAGGTTGCAGCAAGCCGAGATCGCACCATTGCACCCAGGCTGGAGTGCAATGGT30.10362694300518134No Hit
GCTTGCTTTAGGGTGGGGGTAAATATGGCCCCTTCTTTATTTTTATTTTTATATTTATTTATTTAT30.10362694300518134No Hit
GATCAACATTAGGAAATCTGTGAATATAAGTGAGAGCACTGTAGNNNNN30.10362694300518134No Hit
CAATAATTGGGCAGATGAGGATTTTTCGCTTTTTGACTAGTTCTTTCTCT30.10362694300518134No Hit
TGTAAATACACTAATTAAGGGCTTCTTATGCACTAGAAAAAAATGCTTGT30.10362694300518134No Hit
AACAATGGTTAATTTTGATATGTAGTTGTGAACAACTTCAAAACACTTAA30.10362694300518134No Hit
GCCACCGTGCCCGGCCTGTCTTATTTCTTAATTAGCTCCAAATTGGATGT30.10362694300518134No Hit
CATAAAAAATACTCAGAAATTTTGAAAAAATAGAAACAACCACATTCTCA30.10362694300518134No Hit
GCTTATTGCATTTCGTTTGTTGTATCTAATGCTAATCAACTGGTATGTTT30.10362694300518134No Hit
TATCTCTCACCATATATAAAAGTCGGCTCAAAATGATTAAAGACTTTAAAAATAAACCCAATATTATGGCTGGGC30.10362694300518134No Hit
GTTTATTTTTTCTTCTCTCTCAGGTATGCCAATAATTCATGNNNNNNNN30.10362694300518134No Hit
GACACTTGTTAAAAATTGCAAGACTTTATTTGGGATTATTGCAGCAGGGG30.10362694300518134No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGAGA150.00879045130.84848125-129
TTTTTTT500.00765749917.991667105-109
Produced by FastQC (version 0.11.5)