FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2895
Sequences flagged as poor quality0
Sequence length49-150
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATATATATATATATATATATATATATATATATAT140.48359240069084625No Hit
TATTTCCTCTGGCTTCTCTTTTGCAACTTAACTCTCTAACCAGAAGAACA40.1381692573402418No Hit
TGTTCAGATTATTTCTCAGAAGTATTTGTTGAAAATAGCACTTCTGATAA30.10362694300518134No Hit
TTCTTAGTATTGATTTCTATTTTTATTGCACTGTGGTCTGAGAATGTGGT30.10362694300518134No Hit
CTTATGATAAGGTGATGGAGAAGGAAAATGTATGAGCTTGGCGCAGGGAG30.10362694300518134No Hit
AATAAATAAATAAATATAAAAATAAAAATAAAGAAGGGGCCATATTTACCCCCACCCTAAAGCAAG30.10362694300518134No Hit
GTTTAGAATGGGAATTTTTAATACTTTTGGATGATTCAACATTTGAAACT30.10362694300518134No Hit
GCTCAAGGGGGAAACTAGAAGTTTTAAAAAGACAGGAAAGTCTAGAGAAA30.10362694300518134No Hit
CATGAATTATTGGCATACCTGAGAGAGAAGAAAAAATAAACNNNNNNNN30.10362694300518134No Hit
TGCCCAGCCATAATATTGGGTTTATTTTTAAAGTCTTTAATCATTTTGAGCCGACTTTTATATATGGTGAGAGAT30.10362694300518134No Hit
GCCTGTTTTTGCCAACATCACACTGCAGTGTATACCCTGAAAGAGCAATG30.10362694300518134No Hit
GAGGATTGTCCAGCCAAATTCTCTTCAGACACCAAAGCTTCACCCGCTCTCTTCAGGGAG30.10362694300518134No Hit
CCCTGCTTTCTGTAAATTCCTGCTTGCCCCTGGTATATTCCAAGCTGAGT30.10362694300518134No Hit
CATAGGGTTTTAGTCCTGTTTGGATTATATATAACTTCACTCTACCCTTC30.10362694300518134No Hit
CTTAAGTGTTTTGAAGTTGTTCACAACTACATATCAAAATTAACCATTGT30.10362694300518134No Hit
CACCATTGCACTCCAGCCTGGGTGCAATGGTGCGATCTCGGCTTGCTGCAACCTCTGCTTTCTGGATTAAAG30.10362694300518134No Hit
GGAGGCGGAGGCTGCAGTGAGCTGAGATCACGCCACTGCACTCCAGCCTG30.10362694300518134No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA600.00929295217.360216130-134
Produced by FastQC (version 0.11.5)