FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520310
Sequences flagged as poor quality0
Sequence length49-150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGT100.008468151136.63443
CACACCT5452.2790546E-411.281743
CTATGTT6800.00174377319.0419831
ACTCCTG7300.00332183048.421866
CTCCAGC9807.554467E-58.3653721
TCCAGCC10201.2040916E-48.0373182
ATTATCA7900.00676215167.78222476
CTCAGAG7950.00714910267.73402261
CAAACAT9600.00478542887.1163751
CCCAGCT9650.0050333327.0795031
CAAAAGA13052.724668E-46.8055451
CTCAAAA15102.3198326E-46.33404541
CCAGCCT13250.00239572256.1872183
CTTTTTT26202.5883E-65.4758061
Produced by FastQC (version 0.11.5)