FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences831907
Sequences flagged as poor quality0
Sequence length49-150
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGCATAATCTCGTAT8850.10638208357424568TruSeq Adapter, Index 5 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG217900.012.495727140-144
GACACCC3806.215213E-511.006624140-144
AGGGGGG9054.3655746E-119.705289140-144
AAGGGGG9551.2241799E-98.759198140-144
CGGAAGA15900.08.4835024
TCGTATG10701.1878001E-98.208679140-144
TCGGAAG16103.6379788E-128.0678173
ATCGGAA16305.456968E-127.9693042
CGTATGC11102.4301698E-97.9128704140-144
GATCGGA16557.2759576E-127.85222671
GAGCACA16557.2759576E-127.8479799
CCTTGGC5950.0087695747.56036951
AGAGCAC17251.8189894E-117.52950958
CTCGTAT10657.8214725E-87.4617686140-144
TCTCGTA10504.8904803E-77.1700296140-144
ATGCCGT10158.71114E-96.994162130-134
GAAGAGC18557.348717E-106.7333436
GCCGTCT9751.9874278E-86.710995125-129
CGTCTTC10451.727858E-76.705325135-139
CCGTCTT10354.315698E-76.431606135-139
Produced by FastQC (version 0.11.5)