FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1102122
Sequences flagged as poor quality0
Sequence length49-150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG122800.016.434866140-144
TATGCCG10400.012.532111140-144
TGCCGTC10500.012.412758140-144
ATGCCGT10700.011.442517140-144
TCGTATG11450.011.382878140-144
CGTATGC10800.011.336568140-144
GCCGTCT10250.011.174234140-144
CTCGTAT11150.010.980711140-144
GTATGCC13000.010.6333065140-144
AGGGGGG9250.010.247387140-144
TCTCGTA11150.09.918061140-144
CGGGGGG4801.0755748E-59.873785140-144
CGTCTTC10200.09.6801815140-144
ATCTCGT11200.09.185092135-139
CCGTCTT10655.456968E-128.900313140-144
AATCTCG12500.08.530951140-144
CTCCAGC15201.6370905E-118.4110751
AAGGGGG11301.8189894E-118.388348140-144
TGCTTGA13755.0931703E-117.994902140-144
GGGCGGG5100.00209943627.744145140-144
Produced by FastQC (version 0.11.5)