FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1102122
Sequences flagged as poor quality0
Sequence length49-150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGC4953.6379788E-1214.207896140-144
GTGGTCG5700.013.7093725140-144
TCGGTGG5957.2759576E-1212.476683140-144
GCCGTAT3901.0168733E-612.022065140-144
CGGTGGT6453.092282E-1111.509498140-144
GTCGCCG4454.0119266E-711.414208140-144
AGATCGA1900.005563905511.165021130-134
GGGGGGG151650.011.001399140-144
CGCCGTA4001.7637838E-510.744721140-144
CGTATCA4357.777311E-69.880203140-144
TCTCGGT6305.2204996E-109.873488135-139
AGGGGGG7951.382432E-109.829363140-144
GGTGGTC8353.45608E-119.826419140-144
TGTGACG9202.3646862E-119.343236140-144
TCGCCGT3854.6725043E-59.133648140-144
GGTCGCC5253.524057E-58.930677140-144
GTGACGG10101.8189894E-118.897519140-144
GACGGTT9956.730261E-118.638971140-144
TGACGGT9301.1204975E-98.402519140-144
CTCGGTG6052.22701E-58.203051140-144
Produced by FastQC (version 0.11.5)