FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1482724
Sequences flagged as poor quality0
Sequence length49-150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG5804.0738087E-87.745329140-144
CTCGTAT5601.85928E-77.59974140-144
CGTATGC6401.7082948E-66.649772140-144
ATCTCGT5404.0275272E-46.594064140-144
GGGGGGG151600.06.0512733140-144
TATGCCG5250.00169175145.854614140-144
TCTCGTA5859.924922E-45.658306140-144
CTTTAAG17200.0040928035.0546871
Produced by FastQC (version 0.11.5)