FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1482724
Sequences flagged as poor quality0
Sequence length49-150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC3803.1523086E-99.6581335135-139
GCCGTAT3752.3878747E-68.155756135-139
CGTATCA4402.8808845E-67.4143243135-139
GTCGCCG3753.6953894E-57.292458130-134
GGCCAAT8650.00433961867.19017361
GGTCGCC4901.06309104E-46.728949140-144
GTGGTCG5701.5830248E-56.610897140-144
TCGCCGT3550.0011566976.60283130-134
TCTCGGT6207.8553E-66.4576206140-144
TGGTCGC4656.06834E-45.881015130-134
CTCGGTG6701.6606395E-45.624196140-144
CGGTGGT6800.00122231365.1951447140-144
ATCTCGG7406.8121E-45.092178140-144
Produced by FastQC (version 0.11.5)