FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329762

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329762
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222971
Sequences flagged as poor quality0
Sequence length49-150
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATATATATATATATATATATATATATATATATAT9610.4309977530710272No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCATAG1100.002231497622.5473617
GGGGGGG166500.017.240482140-144
TGGGCAA2150.006374300513.8430319
CCCAGGA2800.002526144512.4010491
AGGGGGG8950.011.247345140-144
AAGGGGG8700.09.433304135-139
AAAGGGG9650.08.74898140-144
AAAAGGG10050.08.653226130-134
GCTTGAA13200.07.5607305120-124
AAAAAGG13200.07.503764135-139
CAAAAAA9302.6546424E-57.4672981
CGTCTTC14300.07.0394225140-144
TGCTTGA13350.06.8407784125-129
CTTGAAA14750.06.5970583120-124
GTCTTCT15451.0913936E-116.3052764140-144
ATGCCGT15452.0008883E-116.3052764140-144
CTTCTGC15200.06.2486367115-119
CCGTCTT14650.06.178704110-114
TTCTGCT15450.06.147526115-119
CTGCTTG13550.06.1333485115-119
Produced by FastQC (version 0.11.5)