FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences539992
Sequences flagged as poor quality0
Sequence length49-150
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATATATATATATATATATATATATATATATATAT5590.10352005214892072No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCACGC559.937352E-538.3798681
GGGGGGG235550.025.339317140-144
TGGGCGG1402.5400214E-422.399332140-144
TATCAAG2802.5960145E-422.319977130-134
GCTCGTA900.002535434222.064352125-129
AGGGGGG11350.014.961706130-134
AAGGGGG11650.014.576425130-134
TCGGCCT1750.004767776514.467999
TGGGGGG7650.014.347284140-144
GGGGGGT3302.5529389E-514.254121140-144
GCGGGGG2300.00706283813.6343775140-144
CGGGGGG2850.001953901312.837045140-144
GGGGGTG5158.652805E-712.178278140-144
AAAGGGG12900.011.847583130-134
GTGGGGG7507.307026E-79.756154140-144
AGGCAGT3550.0044704769.5094776
AAAAGGG15550.09.194663125-129
CTTCTGC19900.09.192356140-144
GCTTGAA18150.09.122313130-134
CACCTCT3800.0078235958.8838534
Produced by FastQC (version 0.11.5)