FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1837893
Sequences flagged as poor quality0
Sequence length49-150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG711600.020.030375140-144
AGGGGGG36550.011.5364065140-144
TATGGCG2550.00492978911.347902140-144
TGCCGGG3756.125745E-511.023677140-144
AAGGGGG40400.010.539344140-144
CGTCGGC2350.002141476410.396116125-129
CCGGCGT3650.002480841510.300281140-144
AAAGGGG47400.09.418964140-144
GCCGTCG7251.713488E-99.247191135-139
CGCGTAT6351.8609826E-69.114063140-144
TCTGCGT10550.09.015917130-134
GCGTGAA13450.08.919713135-139
CGGGGGG10802.1827873E-118.803631140-144
GATGCCG5251.2250886E-58.737321135-139
TGCGTGA10201.8189894E-128.648366135-139
CGTCTGC9203.6779966E-98.537358140-144
CGTCGTC7551.6370905E-118.396767115-119
ATGCCGG8602.1827873E-108.390515130-134
AAAAGGG55700.08.386504140-144
GCGGGGG8954.0832674E-88.313946140-144
Produced by FastQC (version 0.11.5)