FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1738977
Sequences flagged as poor quality0
Sequence length49-150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGC6802.7515307E-711.7515881
CGCCGTA4059.122232E-99.857248140-144
CTAGGCC6553.1216678E-49.3825493
GTCGCCG4607.4196578E-99.189174140-144
TCACGCC8404.3059168E-58.779892
TCGCCGT4059.4973075E-78.697572140-144
CACGCCT9903.3227014E-58.069943
GGTCGCC5703.378591E-87.827815140-144
TGGTCGC5652.418492E-77.4814515140-144
GTGGTCG5903.2528733E-67.35433140-144
CGTATCA4951.6460814E-57.116195140-144
ACGCCTG8750.00545375847.0233064
GCCGTAT5152.865161E-56.839838140-144
CACCGGT9254.3655746E-116.368246135-139
CGGTGGT6653.5793437E-66.3564215140-144
CCCAGGA27501.8189894E-106.25874761
CCCCTGT10900.00647892156.2033781
CACAAGT13150.00108124226.0768661
CCACCGG10755.2750693E-115.8575606135-139
AGCGTTT3900.00729912475.600228120-124
Produced by FastQC (version 0.11.5)