FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences887418
Sequences flagged as poor quality0
Sequence length49-150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG295600.018.277292140-144
AGGGGGG16500.011.305761140-144
CCTAACA4355.794993E-410.199891
AAGGGGG17550.09.7653265135-139
AAAGGGG21700.08.758745140-144
TTACAGC5300.0035293018.3668919
CTAAAAC8751.2979897E-57.8879151
CTCAAAA20550.07.6768031
CCCACCA7107.2258885E-47.63794661
CCCAGAA10451.7732214E-57.0764791
CTGCTTG26400.07.0546985130-134
TGCTTGA27750.06.646042140-144
CTCTGAA10401.2692713E-46.6364671
AAAAGGG28150.06.6317368135-139
CTAAAAA18901.0300937E-86.260251
CGTCTTC24700.06.2293496115-119
CCGTCTT24750.06.2167654115-119
ATGCCGT25900.06.1710024120-124
CAAACAC10409.120872E-46.16243361
CTCCTGA10459.6634007E-46.13294841
Produced by FastQC (version 0.11.5)