FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences643118
Sequences flagged as poor quality0
Sequence length49-150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGGC650.003327833721.056229125-129
GGGGGGG205000.018.047363140-144
CTTCGGC1353.999907E-415.8347435135-139
GCCGTCG2352.0767402E-814.319946120-124
GATATGC2200.007435874513.5301127
AGGGGGG11850.012.632846140-144
TGCGTGA3302.0265179E-712.0302925135-139
CCGTCGT2457.37735E-611.622311120-124
GTCGTCT2000.001389450410.949239125-129
CGTCGTC2303.6927455E-410.711211125-129
TGGGGGG7006.91216E-1110.183621140-144
CCCCTTC4507.4371137E-49.9259411
GCGTATG1950.0044857019.507474110-114
AAGGGGG13900.09.447142135-139
TCGTCTG2650.00135654469.296523125-129
CGTGAAA4908.947509E-59.267168140-144
CTCTAGA4350.0062694039.1273011
AAAGGGG16050.09.104981140-144
CTGCGTG3455.5846776E-48.85172135-139
CTCAAAG6251.7019351E-48.7348281
Produced by FastQC (version 0.11.5)