FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329808

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329808
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1753488
Sequences flagged as poor quality0
Sequence length49-150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGAC600.007340896427.8980835
GGGGGGG958150.021.5188140-144
GGGCGTA1153.7819557E-421.12362135-139
AGACTCG1050.004580030719.9272025
CGGGTTC2450.00879926613.191348140-144
AGGGGGG43600.011.835593140-144
AAGGGGG46750.010.545343140-144
GCTCGTA4401.5966398E-610.254478120-124
CGGGGGG9850.09.782715130-134
AAAGGGG54050.09.2915745140-144
AAAAGGG59200.08.398923130-134
CTCGGGA4850.006917637.76455939
TGGGGGG23800.07.743576140-144
AGCTCGT4750.00371107167.6712084135-139
GCTTGAA67050.07.4271336135-139
CTGCTTG66000.07.2999167135-139
TGCTTGA65850.07.1935787135-139
AAAAAGG73300.07.1028557140-144
CGTCTTC63850.07.0628595130-134
CTTGAAA75300.06.613404135-139
Produced by FastQC (version 0.11.5)