FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1753488
Sequences flagged as poor quality0
Sequence length49-150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGCGC1550.001440809317.314226135-139
GGGGGGG655300.014.597742140-144
CCGAGCC1750.00585289514.0301273
GCGGGGG10250.013.635272140-144
CGGCGGG2700.001401117813.420175140-144
GGGCCGT2100.001496175313.304162125-129
AACTCGG1700.002545128612.396791105-109
CGGGGGG11950.012.128695140-144
TGGGGGG31150.010.801379140-144
AGGGGGG23250.010.773296135-139
GAGGGCG2500.0087716110.501461120-124
GGCGGGG10501.2732926E-1110.352706140-144
CGCCGTA17050.09.968797135-139
AAGGGGG20750.09.915697135-139
CCGTATC17250.09.853216135-139
TCGCCGT16800.09.850901135-139
GCCGTAT17000.09.735008135-139
GGTCGCC18500.09.605025130-134
CGTATCA17750.09.332017140-144
AACCCCG3550.00577739079.2201017
Produced by FastQC (version 0.11.5)