FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences682350
Sequences flagged as poor quality0
Sequence length49-150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG84050.019.894907140-144
TGCCGTC5001.7516868E-915.054878140-144
TCGGCCT1950.00475596314.4741773
TATGCCG4952.4881956E-814.120737140-144
CTCGGCC2000.005625919414.1123232
CCTCGGC2100.007763785313.4422791
GCCGTCT4602.380868E-612.857428140-144
ATGCCGT5054.851154E-712.776417140-144
CGTCTTC4652.6876369E-612.719175140-144
CCGTCTT4703.0297306E-612.583865140-144
CGTATGC5401.091641E-611.948316140-144
CGGGGGG3600.0032898399.869845135-139
GTATGCC6306.2610707E-79.869844135-139
AGGGGGG4851.0047639E-49.16924130-134
TCGTATG4903.5596662E-59.075675130-134
TATCTCG4800.00368051258.961237140-144
AAGGGGG6055.357833E-48.887177140-144
CTCGTAT4750.00181343528.415341135-139
TCTCGTA4850.00101405678.335672130-134
CTCCAGG6303.3058936E-48.21472551
Produced by FastQC (version 0.11.5)