FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences682350
Sequences flagged as poor quality0
Sequence length49-150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTAAC958.917243E-657.70724140-144
GCCGTAA800.00653797528.564604140-144
AGAGCGG2201.3935148E-418.177475140-144
GGGGGGG135650.017.08701140-144
CGTATCA6150.014.714203135-139
CGCCGTA6400.013.56996130-134
CCGTATC5801.8189894E-1213.4745655135-139
GTCGCCG6750.012.866332130-134
AGGGGGG8101.8189894E-1212.228834140-144
GCCGTAT6455.456968E-1211.708492130-134
GTGGTCG7450.011.65138125-129
TGGTCGC6700.011.400961125-129
TCGCCGT6102.6193447E-1011.1422615130-134
GGTCGCC6857.2759576E-1211.024785130-134
CGGTGGT7300.010.939528125-129
TCTCGGT7553.6379788E-129.669517120-124
GTATCAT7905.2532414E-99.372049135-139
AAGGGGG7753.8331564E-89.022697135-139
GGGGGGT6051.2227014E-49.0048685140-144
GATCTCG7805.638867E-118.952657120-124
Produced by FastQC (version 0.11.5)